Part:BBa_K2616000:Design

proNGF and TEV secretion via E. coli Type I Secretion System

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 62
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 430
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1845
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1831
  

Design Notes

Codons were optimized for E. coli.

Source

Rattus Norvegicus Nerve Growth Factor: NCBI Reference Sequence: NM_001277055.1 TEV Protease : Part:BBa_K1319004 HlyA export sequence: Part:BBa_K554002